PYCC 10015
1 - Taxon name
Cyberlindnera hibernica
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Cyberlindnera hibernica
5 - Basis for identification
Molecular (whole genome sequence)
6 - Identified by
Kenneth Wolfe
7 - Original strain number
UCD1070
8 - Accession numbers in other collections
NA
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Soil under an oak tree
14 - Category of substrate
Soil
15 - Locality
Glengarriff Woods, Co. Cork, Ireland
16 - Country of origin
Ireland
17 - Latitude, longitude coordinates

51.75708, -9.56761

18 - Sample Collected by
Geraldine Butler
19 - Isolated by and date of isolation
Julianna Decuseara, September 2023
20 - Isolation details
NA
21 - Deposited by
Ken Wolfe, Mai 2024
22 - History
Ken Wolfe > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
Whole genome
SAMN44006581
F1 D-Glucose
+
F2 D-Galactose
-
F3 Maltose
-
F4 Me a-D-Glucoside
-
F5 Sucrose
+
F6 a,a-Trehalose
-
F7 Melibiose
-
F8 Lactose
-
F9 Cellobiose
-
F10 Melezitose
-
F11 Raffinose
-
C1 D-Glucose
+
C2 D-Galactose
-
C3 L-Sorbose
-
C4 D-Glucosamine
-
C5 D-Ribose
-
C6 D-Xylose
+
C7 L-Arabinose
-
C8 D-Arabinose
-
C9 L-Rhamnose
-
C10 Sucrose
+
C11 Maltose
W
C12 a,a-Trehalose
-
C13 Me a-D-Glucoside
-
C14 Cellobiose
+
C15 Salicin
+
C18 Lactose
-
C19 Raffinose
+
C20 Melezitose
-
C21 Inulin
-
C22 Starch
W
C23 Glycerol
+
C24 Erythritol
-
C25 Ribitol
-
C26 Xylitol
+
C28 D-Glucitol
+
C29 D-Mannitol
+
C30 Galactitol
D
C31 myo-Inositol
-
C32 D-Glucono-1,5-lactone
-
C34 5-Keto-D-Gluconate
+
C35 D-Gluconate
-
C38 DL-Lactate
+
C39 Succinate
-
C40 Citrate
-
C41 Methanol
-
C42 Ethanol
+
C50 L-Malic acid
+
C51 L-Tartaric acid
-
N1 Nitrate
-
N2 Nitrite
-
N3 Ethylamine
+
N4 L-Lysine
+
N5 Cadaverine
+
N6 Creatine
-
N7 Creatinine
-
V1 w/o vitamins
-
T1 Growth at 25ºC
+
T3 Growth at 35ºC
-
T4 Growth at 37ºC
-
O1 Cycloheximide 0.01%
-
O2 Cycloheximide 0.1%
-
O6 10% NaCl
+
Title
Cyberlindnera hibernica sp. nov. and Barnettozyma discipulorum sp. nov., isolated from forest soil in Ireland

Author

Adam P. Ryan, Cláudia Carvalho, Yiran Zhao, Julianna Decuseara, Matthieu Osborne, Padraic G. Heneghan, Kevin P. Byrne, Tadhg Ó Cróinín, Kenneth H. Wolfe, José Paulo Sampaio, Geraldine Butler

Abstract

Two yeast strains, PYCC 10015 and PYCC 10016, were isolated from soil from an Irish forest. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rRNA gene repeat, and the D1/D2 domain of the LSU rRNA gene, showed that they belong to the Cyberlindnera and Barnettozyma genera of the order Phaffomycetales, but they did not exactly match any known species. The genomes of both isolates were sequenced using Oxford Nanopore Technologies and Illumina sequencing, generating chromosome-level genome assemblies. Phylogenomic analysis of 1,385 single-copy orthologues from 37 Phaffomycetales species and 2 outgroups showed that the closest relative of PYCC 10015 is Cyberlindnera galapagoensis and that PYCC 10016 is placed in a subclade containing 7 other species from the Barnettozyma genus. The average nucleotide identity between these strains and their closest relatives is <75%, supporting their designation as novel species. Here, we propose the names Cyberlindnera hibernica sp. nov. and Barnettozyma discipulorum sp. nov. for PYCC 10015 and PYCC 10016, respectively.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.006898
Publication Pdf