PYCC 10042
1 - Taxon name
Vishniacozyma siamensis
2 - Classification
Basidiomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Vishniacozyma siamensis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Pajongwan Gungprakhon et. al. 2025
7 - Original strain number
DMKU-SG26
8 - Accession numbers in other collections
TBRC 18499
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Leaf of Rhizophora sp.
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
Mangrove forest, Chanthaburi
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
Pajongwan Gungprakhon
20 - Isolation details
04/24/2023
21 - Deposited by
Sasitorn Jindamorakot, Oct. 2024
22 - History
Sasitorn Jundamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
MYP
DNA Sequence
Region
26S
Sequence Title
LC797015
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/LC797015.1
DNA Sequence
Region
ITS
Sequence Title
LC786857
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/LC786857.1
Title
Vishniacozyma siamensis sp. nov., a new anamorphic tremellomycetous yeast species isolated from a mangrove forest in Thailand

Author

Pajongwan Gungprakhon, Maneerat Khammeankea, Savitree Limtong, Pannida Khunnamwong

Abstract

Six strains (DMKU-SG26, DMKU-SG42, DMKU-SYM22, DMKU-RG41, DMKU-RX317 and DMKU-RGM25) representing a novel basidiomycetous yeast species were isolated from leaf surfaces of mangrove plants collected in Thailand. Pairwise sequence analysis indicated that the six strains either had identical nucleotide substitution in the D1/D2 domains of the large subunit (LSU) rRNA gene sequences or differed by one to three nucleotide(s). They also had identical or differed by one to five nucleotide substitution(s) in the internal transcribed spacer (ITS) regions. blastn searches of the GenBank database revealed that the six strains were closely related to the holotype of type strains of Vishniacozyma peneaus, V. terrae, V. phoenicis, V. taiwanica and V. europaea, but with 6–15 (1.14–2.48%) and 16–26 (5.4–8.8%) nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and the ITS regions, respectively. Phylogenetic analysis based on the concatenated sequences of the ITS regions and D1/D2 domains of the LSU rRNA gene showed that these strains are placed in the Vishniacozyma clade but were at a distinctly different position from the other recognized species of the genus. Based on the phylogenetic analysis and phenotypic characteristics, these six strains are a novel species of the genus Vishniacozyma, for which the name Vishniacozyma siamensis sp. nov. is proposed to accommodate them. The holotype is TBRC 18499T and the ex-type culture is PYCC 10042 (=DMKU-SG26). The MycoBank number of the novel species is MB 855838.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.006623
Publication Pdf