PYCC 10152
1 - Taxon name
Yamadazyma oleae
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Yamadazyma oleae
5 - Basis for identification
Molecular (D1/D2; ITS)
6 - Identified by
M. Avesani and G. Zapparoli
7 - Original strain number
CO-CAV2
8 - Accession numbers in other collections
CBS 18661
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
Yes
13 - Substrate of isolation
Stored olive oil
14 - Category of substrate
Food & beverages
15 - Locality
Cavaion Veronese, Verona
16 - Country of origin
Italy
17 - Latitude, longitude coordinates

45.529975, 10.75299

18 - Sample Collected by
M. Avesani
19 - Isolated by and date of isolation
M. Avesani and G. Zapparoli, November 2023
20 - Isolation details
NA
21 - Deposited by
M. Avesani & G. Zapparoli, Feb. 2025
22 - History
M. Avesani > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
PP130150
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/PP130150
DNA Sequence
Region
ITS
Sequence Title
PP149061
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/PP149061
Title
Yamadazyma oleae f.a. sp. nov. and Yamadazyma molendinolei f.a. sp. nov., two novel ascomycetous yeast species isolated from olive oil mills in Italy, and reassignment of 11 Candida species to the genus Yamadazyma

Author

Michele Avesani, Giacomo Zapparoli, Sasitorn Jindamorakot, Savitree Limtong

Abstract

A corrigendum of this article has been published full details can be found at 10.1099/ijsem.0.006866 Three yeast strains were isolated from an olive paste sample (CBS 18661) and two samples of olive oil (CBS 18662 and CBS 18660) were collected in two different mills in Verona province, Italy. The sequence comparison of the D1/D2 domains of the LSU rRNA gene and the internal transcribed space (ITS) regions indicated that these strains belonged to the genus Yamadazyma. Phylogenetic analysis of concatenated sequences of the ITS regions and the D1/D2 domains indicated that they represented members of two distinct species. Strain CBS 18661 and CBS 18662 showed sequence divergence of 2.7–2.8% (13–15 nucleotide substitutions) in the D1/D2 domains from the holotype of Yamadazyma luoyangensis, Yamadazyma ovata, Yamadazyma siamensis and Candida kanchanaburiensis and 5.9–6.2% identities (36–38 nucleotide substitutions) in the ITS regions from Yamadazyma akitaenisis and Yamadazyma nakazawae. Strain CBS 18660 showed sequence divergence of 0.75–1.1% (4–6 nucleotide substitutions) from Y. ovata, Candida trypodendri and Candida insectorum and 3.2–4.1% (18–24 nucleotide substitutions) in the ITS regions from Yamadazyma dushanensis, Yamadazyma terventina, Yamadazyma mexicana and C. trypodendri. The strains CBS 18661T (PP391581-PP375117) and CBS 18662 (PP391582-PP375118) are named Yamadazyma oleae f.a. sp. nov., whereas CBS 18660T (PP149061-PP130150) is assigned as Yamadazyma molendinolei f.a. sp. nov. The MycoBank numbers are MB 854683 and MB 854684, respectively. In addition, 11 Candida species belonging to the Yamadazyma clade have been reassigned to the genus Yamadazyma with the proposal of the following new combinations: Yamadazyma aaseri comb. nov., Yamadazyma conglobata comb. nov., Yamadazyma dendronema comb. nov., Yamadazyma diddensiae comb. nov., Yamadazyma germanica comb. nov., Yamadazyma insectorum comb. nov., Yamadazyma kanchanaburiensis comb. nov., Yamadazyma naeodendra comb. nov., Yamadazyma pseudoaaseri comb. nov., Yamadazyma trypodendri comb. nov. and Yamadazyma vaughaniae comb. nov.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.006592
Publication Pdf