PYCC 8347
1 - Taxon name
Cryolevonia schafbergensis
2 - Classification
Basidiomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Cryolevonia schafbergensis
5 - Basis for identification
Molecular (D1D2)
6 - Identified by
Joel Ruethi and Beat Frey
7 - Original strain number
414
8 - Accession numbers in other collections
CCOS 1156; CBS 16055
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
12000 year old permafrost soil collected at 2960 m altitude (soil depth of 160 cm)
14 - Category of substrate
Soil
15 - Locality
mountain ridge ‘Muot da Barba Peider’ south-west of Piz Muragl and north-east of Pontresina in the upper Engadine valley (Eastern Swiss Alps)
16 - Country of origin
Switzerland
18 - Sample Collected by
Joel Ruethi and Beat Frey (September 2014)
19 - Isolated by and date of isolation
NA
20 - Isolation details
NA
21 - Deposited by
A. Pontes, May 2019
22 - History
A.Pontes > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150ºC
24 - Price per culture
85€
25 - Remarks
Recommended growth temperature: 4ºC
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
MN058075
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MN058075
DNA Sequence
Region
ITS
Sequence Title
MN058074
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MN058074
C1 D-Glucose
+
C2 D-Galactose
-
C3 L-Sorbose
-
C4 D-Glucosamine
-
C5 D-Ribose
-
C6 D-Xylose
-
C7 L-Arabinose
-
C8 D-Arabinose
-
C9 L-Rhamnose
-
C10 Sucrose
+
C11 Maltose
+
C12 a,a-Trehalose
-
C13 Me a-D-Glucoside
-
C14 Cellobiose
-
C15 Salicin
+
C17 Melibiose
-
C18 Lactose
-
C19 Raffinose
-
C20 Melezitose
+
C21 Inulin
-
C23 Glycerol
D
C24 Erythritol
-
C25 Ribitol
-
C26 Xylitol
-
C28 D-Glucitol
W
C29 D-Mannitol
+
C30 Galactitol
-
C31 myo-Inositol
-
C32 D-Glucono-1,5-lactone
-
C35 D-Gluconate
D
C36 D-Glucuronate
-
C38 DL-Lactate
-
C39 Succinate
W
C40 Citrate
-
C41 Methanol
+
C42 Ethanol
+
C50 L-Malic acid
-
C51 L-Tartaric acid
-
N1 Nitrate
+
N2 Nitrite
-, +
N3 Ethylamine
-
N4 L-Lysine
-
N5 Cadaverine
-
N6 Creatine
-
N7 Creatinine
-
V1 w/o vitamins
+
T1 Growth at 25ºC
-
O1 Cycloheximide 0.01%
-
O2 Cycloheximide 0.1%
-
O6 10% NaCl
-
P1 Protocatechuic acid
-
Title
Cryolevonia gen. nov. and Cryolevonia schafbergensis sp. nov., a cryophilic yeast from ancient permafrost and melted sea ice

Author

Ana Pontes, Joel Ruethi, Beat Frey, Andreia Aires, Amanda Thomas, David Overy, Brad Halti, Russell Kerr, José Paulo Sampaio

Abstract

A cryophilic basidiomycetous yeast unable to grow at 18 °C or higher temperatures was isolated from a subsurface permafrost layer collected in the Eastern Swiss Alps and from melted sea ice collected in the Artic at Frobisher Bay, Nunavut, Canada. Phylogenetic analyses employing combined sequences of the D1/D2 domain and ITS region indicated that the two new isolates belong to the family Camptobasidiaceae of the class Microbotryomycetes but are distantly related to any of the currently recognized species and genera. Consequently, the novel genus Cryolevonia, and the novel species Cryolevonia schafbergensis (type strain PYCC 8347T=CBS 16055T) are proposed to accommodate this cryophilic yeast. Although sparse hyphae and teliospore-like stuctures were observed upon prolonged incubation, a sexual cycle was not observed and therefore C. schafbergensis is documented solely from its asexual stage.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.004040