PYCC 9794
1 - Taxon name
Savitreea siamensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Savitreea siamensis
5 - Basis for identification
Molecular (D1D2)
6 - Identified by
Pannida Khunnamwong et al 2024
7 - Original strain number
ST-3660
8 - Accession numbers in other collections
TBRC 4481
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Estuarine water
14 - Category of substrate
Water
15 - Locality
Laem Son National Park, Suk Samran, Ranong
16 - Country of origin
Thailand
18 - Sample Collected by
Unknown
19 - Isolated by and date of isolation
Sasitorn Jindamorakot, 29/05/2012
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, Jul. 2023
22 - History
Sasitorn Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
Title
Savitreea siamensis sp. nov., an ascomycetous yeast species in the family Saccharomycetaceae discovered in Thailand

Author

Pannida Khunnamwong, Sasitorn Jindamorakot, Somjit Am-In, Varunya Sakpuntoon, Nantana Srisuk, Pumin Nutaratat, Wanatchaporn Boontham, Savitree Limtong

Abstract

Four yeast strains, representing a novel anamorphic species, were isolated in Thailand. The two strains (ST-3660T and ST-3647) were obtained from two different estuarine water samples in a mangrove forest. Strain DMKU-FW1-37 was derived from a grease sample, and another strain (TSU57) was isolated from a fruiting body of Phallus sp. Pairwise sequence analysis showed that the four strains had identical or differed by only one nucleotide substitution in the D1/D2 domains of the large subunit (LSU) rRNA gene, and differed by one to three nucleotide substitutions in the internal transcribed spacer (ITS) regions. Savitreea pentosicarens is the most closely related species to the four strains, but with 9–10 (1.57–1.72 %) nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 29–31 (4.22–4.45 %) nucleotide substitutions in the ITS regions. Phylogenetic analyses based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene showed that the four strains form a well-separated lineage from S. pentosicarens with high bootstrap support, confirming that they represent a distinct species. Therefore, the four strains are assigned as representives of a novel species of the genus Savitreea, for which the name Savitreea siamensis sp. nov. is proposed. The holotype is TBRC 4481T and the ex-type is PYCC 9794T (=ST-3660T). The MycoBank number of the novel species is MB 851951.

Publication Date

Link to Publication

http://dx.doi.org/10.1099/ijsem.0.006330
Publication Pdf