PYCC 10388
1 - Taxon name
Kwoniella olivae
2 - Classification
Basidiomycota
3 - Strain (species name) changes
Kwoniella sp.
4 - Status of the strain
NA
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Avesani et al 2025
7 - Original strain number
CAB 1573
8 - Accession numbers in other collections
NA
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Insect frass on olive trees
14 - Category of substrate
Animal
15 - Locality
Stellenbosch
16 - Country of origin
South Africa
17 - Latitude, longitude coordinates

-33.935398, 18.874327

18 - Sample Collected by
2019
19 - Isolated by and date of isolation
2019
20 - Isolation details
NA
21 - Deposited by
Alfred Botha Jul. 2025
22 - History
Alfred Botha > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
MYP
DNA Sequence
Region
26S
Sequence Title
MN599183
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MN599183
DNA Sequence
Region
ITS
Sequence Title
PV205206
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/PV205206
Title
Kwoniella olivae f.a. sp. nov., a novel basidiomycetous yeast species associated with olive ecosystems

Author

Michele Avesani, Claudia Carvalho, Cleo Gertrud Conacher, Heidi Christa Steffen, Alfred Botha, Giacomo Zapparoli, Jose Paulo Sampaio

Abstract

Three yeast strains of the basidiomycetous genus Kwoniella (subphylum Agaricomycotina; class Tremellomycetes; subclass Tremellomycetidae) were isolated from samples of olive oil collected in northern Italy. Analysis of the D1/D2 domains of the 26S rRNA gene (LSU) and of the complete internal transcribed spacer (ITS) region indicated that these strains displayed high sequence similarity to three other strains isolated from insect frass in olive trees in South Africa, suggesting that they are conspecific. Phylogenetic analyses based on LSU and ITS sequences placed these six strains in a well-supported clade closely related to Kwoniella newhampshirensis, Kwoniella shandongensis and Kwoniella lonicerae with sequence divergence of 1.2–1.6% (7–9 nucleotide substitutions) in the D1/D2 domains and 1.8–6.4% (12–37 nucleotide substitutions) in the ITS regions. Based on these results and on phenotypic analyses, a novel species, Kwoniella olivae f.a. sp. nov. PYCC 9971T (=CBS 18666T), is proposed. The MycoBank number is MB 860006.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.006916
Publication Pdf