PYCC 2972
1 - Taxon name
Wickerhamiella versatilis
2 - Classification
Ascomycota
3 - Strain (species name) changes
Candida versatilis; Debaryomyces tamarii
4 - Status of the strain
Type strain of Debaryomyces tamarii
5 - Basis for identification
Molecular (D1D2)
6 - Identified by
A. Pontes, Jun. 2023
7 - Original strain number
NA
8 - Accession numbers in other collections
CBS 4333;CCRC 22424;IFO 0854;JCM 6351;NRRL Y-6665
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
13 - Substrate of isolation
mash of tamari-soya
14 - Category of substrate
Food & beverages
15 - Locality
Unknown
16 - Country of origin
Unknown
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
NA
20 - Isolation details
NA
21 - Deposited by
CBS 4333, 1961
22 - History
CBS > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150ºC
24 - Price per culture
85€
25 - Remarks
Molecular identification (D1D2) did not correspond to the original identification - Schwanniomyces vanrijiae
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
U94920
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/U94920
F1 D-Glucose
-
C1 D-Glucose
+
C2 D-Galactose
+
C3 L-Sorbose
D
C4 D-Glucosamine
W
C5 D-Ribose
W
C6 D-Xylose
D
C7 L-Arabinose
D
C8 D-Arabinose
-
C9 L-Rhamnose
-
C10 Sucrose
+
C11 Maltose
W
C12 a,a-Trehalose
+
C13 Me a-D-Glucoside
W
C14 Cellobiose
+
C15 Salicin
W
C16 Arbutin spliting
+
C17 Melibiose
-
C18 Lactose
W
C19 Raffinose
-
C20 Melezitose
-
C21 Inulin
-
C22 Starch
W
C23 Glycerol
D
C24 Erythritol
-
C25 Ribitol
-
C28 D-Glucitol
-
C29 D-Mannitol
+
C30 Galactitol
W
C31 myo-Inositol
-
C32 D-Glucono-1,5-lactone
-
C35 D-Gluconate
+
C36 D-Glucuronate
-
C38 DL-Lactate
-
C39 Succinate
D
C40 Citrate
-
C41 Methanol
-
C42 Ethanol
W, D +
C50 L-Malic acid
-
C51 L-Tartaric acid
-
N1 Nitrate
-
N2 Nitrite
-
N3 Ethylamine
W
N4 L-Lysine
W
N5 Cadaverine
W
N6 Creatine
W
N7 Creatinine
D
V1 w/o vitamins
-
T1 Growth at 25ºC
+
T1 Growth at 30ºC
+
T3 Growth at 35ºC
+
T4 Growth at 37ºC
+
T5 Growth at 40ºC
-
O1 Cycloheximide 0.01%
-
O2 Cycloheximide 0.1%
-
O4 50% D-Glucose
+
O6 10% NaCl
-
M1 Starch formation
-
M2 Acid acetic production
-
M3 Urea hydrolysis
-
M4 Diazonium Blue B reaction
-
Title
Flowers as a reservoir of yeast diversity: description of Wickerhamiella nectarea f.a. sp. nov., and Wickerhamiella natalensis f.a. sp. nov. from South African flowers and pollinators, and transfer of related Candida species to the genus Wickerhamiella as new combination