PYCC 2995
1 - Taxon name
Torulaspora ventriculi
2 - Classification
Ascomycota
3 - Strain (species name) changes
Torulaspora globosa, Debaromyces globosa
4 - Status of the strain
Type strain of Torulaspora ventriculi
5 - Basis for identification
Molecular (whole genome sequence)
6 - Identified by
Margarida Silva
7 - Original strain number
Hp 41
8 - Accession numbers in other collections
CBS 4887
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
faeces of Man
14 - Category of substrate
Human
15 - Locality
Vila Cabral
16 - Country of origin
Mozambique
18 - Sample Collected by
N van Uden
19 - Isolated by and date of isolation
N van Uden
20 - Isolation details
NA
21 - Deposited by
N van Uden, 1961
22 - History
N van Uden > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150ºC
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
MK660250
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MK660250
Whole genome
GCA_964263485
F1 D-Glucose
+
F2 D-Galactose
-
F3 Maltose
-
F5 Sucrose
W, D
F6 a,a-Trehalose
-
F7 Melibiose
-
F8 Lactose
-
F9 Cellobiose
-
F10 Melezitose
-
F11 Raffinose
-
C1 D-Glucose
+
C2 D-Galactose
-
C3 L-Sorbose
-
C4 D-Glucosamine
-
C5 D-Ribose
-
C6 D-Xylose
-
C7 L-Arabinose
-
C8 D-Arabinose
-
C9 L-Rhamnose
-
C10 Sucrose
+
C11 Maltose
-
C12 a,a-Trehalose
-
C13 Me a-D-Glucoside
-
C14 Cellobiose
-
C15 Salicin
-
C17 Melibiose
-
C18 Lactose
-
C19 Raffinose
+
C20 Melezitose
-
C21 Inulin
-
C22 Starch
-
C23 Glycerol
+
C24 Erythritol
-
C25 Ribitol
W
C26 Xylitol
-
C28 D-Glucitol
-
C29 D-Mannitol
-
C30 Galactitol
-
C31 myo-Inositol
-
C32 D-Glucono-1,5-lactone
-
C35 D-Gluconate
-
C36 D-Glucuronate
-
C38 DL-Lactate
+
C39 Succinate
-
C40 Citrate
-
C41 Methanol
-
C42 Ethanol
+
C50 L-Malic acid
-
C51 L-Tartaric acid
-
N1 Nitrate
-
N2 Nitrite
-
N3 Ethylamine
-
N4 L-Lysine
D
N5 Cadaverine
-
N6 Creatine
-
N7 Creatinine
-
V1 w/o vitamins
+
T1 Growth at 25ºC
+
T1 Growth at 30ºC
+
T3 Growth at 35ºC
+
T4 Growth at 37ºC
+
T5 Growth at 40ºC
+
T6 Growth at 42ºC
-
T7 Growth at 45ºC
-
O1 Cycloheximide 0.01%
+
O2 Cycloheximide 0.1%
D
P1 Protocatechuic acid
-
M1 Starch formation
-
M3 Urea hydrolysis
-
Title
A taxogenomic view of the genus Torulaspora: an expansion from ten to twenty-two species

Author

M.R. Silva, F. Paraíso, J. Al-Oboudi, M. Abegg, A. Aires, K.O. Barros, P.H. Brito, M. Jarzyna, K. Sylvester, Q.K. Langdon, D.A. Opulente, F. Carriconde, J.W. Fell, T.A. Hofmann, M.-A. Lachance, J.-L. Legras, D. Libkind, A. Pontes, P. Gonçalves, C.A. Rosa, M. Groenewald, C.T. Hittinger, J.P. Sampaio

Abstract

The yeast genus Torulaspora (subphylum Saccharomycotina, family Saccharomycetaceae) is mostly known from its type species, T. delbrueckii, a frequent colonizer of wine and sourdough bread fermentations. The genus currently contains 10 species that are typically found in various natural terrestrial environments in temperate and tropical climates. Here we employ taxogenomic analyses to investigate a large collection of Torulaspora strains obtained in multiple surveys we carried out in Asia, Australasia, North America, South America, and Europe, and to which we added several strains maintained in culture collections. Our analyses detected twelve novel species that are formally described here, thereby more than doubling the species diversity of Torulaspora. We also sketch a genotype-phenotype map for the genus and show how the complex relationship between key genes and the physiological traits they control both between and within species. This remarkable increase in the number of species in the genus Torulaspora highlights how limited the current inventory of fungal taxa is. It also shows how integrated taxogenomic approaches can foster the assessment of species circumscriptions in fungi.

Publication Date

Link to Publication

https://doi.org/10.3114/persoonia.2025.54.08