PYCC 8104
1 - Taxon name
Rhodosporidiobolus oreadorum
2 - Classification
Basidiomycota
3 - Strain (species name) changes
None
4 - Status of the strain
NA
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
J.P. Sampaio
7 - Original strain number
ZP 295
8 - Accession numbers in other collections
NA
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
Yes
13 - Substrate of isolation
soil under snow
14 - Category of substrate
Soil
15 - Locality
Serra da Estrela
16 - Country of origin
Portugal
18 - Sample Collected by
J.P. Sampaio, March 1992
19 - Isolated by and date of isolation
J.P. Sampaio, March 1992
20 - Isolation details
NA
21 - Deposited by
J.P. Sampaio, Mar. 2018
22 - History
J.P. Sampaio > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150ºC
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
JN246538
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/JN246538
DNA Sequence
Region
ITS
Sequence Title
JN246561
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/JN246561
Title
Cystobasidium alpinum sp. nov. and Rhodosporidiobolus oreadorum sp. nov. from European Cold Environments and Arctic Region

Author

Benedetta Turchetti, Laura Selbmann, Nina Gunde-Cimerman, Pietro Buzzini, José Sampaio, Polona Zalar

Abstract

Over 80% of the Earth’s environments are permanently or periodically exposed to temperatures below 5 °C. Cold habitats harbour a wide diversity of psychrophilic and psychrotolerant yeasts. During ecological studies of yeast communities carried out in cold ecosystem in the Italian Alps, Svalbard (Norway, Arctic region), and Portugal, 23 yeast strains that could not be assigned to any known fungal taxa were isolated. In particular, two of them were first identified as Rhodotorula sp., showing the highest degree of D1/D2 sequence identity with Cystobasidum laryngis accounted to only 97% with the type strain (C. laryngis CBS 2221). The other 21 strains, exhibiting identical D1/D2 sequences, had low identity (97%) with Rhodosporidiobolus lusitaniae and Rhodosporidiobolus colostri. Similarly, ITS sequences of the type strains of the most closely related species (93–94%). In a 2-genes multilocus D1/D2 and ITS ML phylogenetic tree, the studied strains pooled in two well separated and supported groups. In order to classify the new 23 isolates based on phylogenetic evidences, we propose the description of two novel species Cystobasidium alpinum sp. nov. and Rhodosporidiobolus oreadorum sp. nov.

Publication Date

Link to Publication

https://doi.org/10.3390/life8020009
Publication Pdf