PYCC 8129
1 - Taxon name
Saccharomyces cerevisiae
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
NA
5 - Basis for identification
Molecular (whole genome sequence)
6 - Identified by
Almeida et al. 2015
7 - Original strain number
ZP 736
8 - Accession numbers in other collections
NA
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
Yes
13 - Substrate of isolation
Rotten figs
14 - Category of substrate
Food & beverages
15 - Locality
Caratão, Abrantes
16 - Country of origin
Portugal
18 - Sample Collected by
E. Valério, Aug. 2008
19 - Isolated by and date of isolation
P. Almeida, Sept. 2008
20 - Isolation details
Saccharomyces selective enrichment protocol (Sampaio and Gonçalves, 2008) and incubation at 30 °C
21 - Deposited by
J.P. Sampaio, Nov. 2018
22 - History
J.P. Sampaio > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150ºC
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
Whole genome
ERX696615
Title
A population genomics insight into the Mediterranean origins of wine yeast domestication

Author

Pedro Almeida, Raquel Barbosa, Polona Zalar, Yumi Imanishi, Kiminori Shimizu, Benedetta Turchetti, Jean‐Luc Legras, Marta Serra, Sylvie Dequin, Arnaud Couloux, Julie Guy, Douda Bensasson, Paula Gonçalves, José Paulo Sampaio

Abstract

AbstractThe domestication of the wine yeast Saccharomyces cerevisiae is thought to be contemporary with the development and expansion of viticulture along the Mediterranean basin. Until now, the unavailability of wild lineages prevented the identification of the closest wild relatives of wine yeasts. Here, we enlarge the collection of natural lineages and employ whole‐genome data of oak‐associated wild isolates to study a balanced number of anthropic and natural S. cerevisiae strains. We identified industrial variants and new geographically delimited populations, including a novel Mediterranean oak population. This population is the closest relative of the wine lineage as shown by a weak population structure and further supported by genomewide population analyses. A coalescent model considering partial isolation with asymmetrical migration, mostly from the wild group into the Wine group, and population growth, was found to be best supported by the data. Importantly, divergence time estimates between the two populations agree with historical evidence for winemaking. We show that three horizontally transmitted regions, previously described to contain genes relevant to wine fermentation, are present in the Wine group but not in the Mediterranean oak group. This represents a major discontinuity between the two populations and is likely to denote a domestication fingerprint in wine yeasts. Taken together, these results indicate that Mediterranean oaks harbour the wild genetic stock of domesticated wine yeasts.

Publication Date

Link to Publication

https://doi.org/10.1111/mec.13341