PYCC 8341
1 - Taxon name
Candida cabralensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Candida cabralensis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
C. Carvalho
7 - Original strain number
NA
8 - Accession numbers in other collections
CBS 11679; CECT 13027; MUCL 55437; MUCL 55437; NCYC 3812
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
Yes
13 - Substrate of isolation
Spanish blue-veined Cabrales cheese
14 - Category of substrate
Food & beverages
15 - Locality
Tielve, Asturias
16 - Country of origin
Spain
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
Álvarez-Martín et al.
20 - Isolation details
NA
21 - Deposited by
CECT, Apr. 2019
22 - History
Belloch, C. & Mayo, B. > CECT > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150ºC
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
NG_055163
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/NG_055163.1
Title
Candida cabralensis sp. nov., a yeast species isolated from traditional Spanish blue-veined Cabrales cheese

Author

Ana Belén Flórez, Carmela Belloch, Pablo Álvarez-Martín, Amparo Querol, Baltasar Mayo

Abstract

Three yeast strains, 1AD8T, 3AD15 and 3AD23, belonging to a previously unknown yeast species were isolated from two independent batches of the Spanish blue-veined Cabrales cheese, a traditional cheese manufactured without the addition of starter and mould cultures. Physiological characterization revealed that the unknown yeast is not fermentative and does not assimilate lactose; rather it assimilates dl-lactic acid and ethanol, major end products of lactic acid bacteria metabolism in cheese. The novel yeast is anamorphic. Phylogenetic tree reconstruction based on nucleotide sequence comparison of the D1/D2 region of the 26S rRNA gene showed that Pichia terricola and Pichia fermentans are the closest relatives of the unknown species. The name Candida cabralensis sp. nov. is proposed, and the isolate 1AD8T (=CECT 13027T =CBS 11679T) is the type strain of this novel taxon.

Publication Date

Link to Publication

https://doi.org/10.1099/ijs.0.017848-0