PYCC 8358
1 - Taxon name
Limtongella siamensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Limtongella siamensis
5 - Basis for identification
Molecular (D1D2)
6 - Identified by
Nantana Srisuk
7 - Original strain number
DMKU-JMGT1-45
8 - Accession numbers in other collections
TBRC 10242
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Grease trap
14 - Category of substrate
Other
15 - Locality
Bangkok
16 - Country of origin
Thailand
18 - Sample Collected by
Jirameth Angchuan, Aug. 2017
19 - Isolated by and date of isolation
NA
20 - Isolation details
YPD with sodium propionate and chloramphenicol, room temperature
21 - Deposited by
Nantana Srisuk, Sept. 2019
22 - History
N. Srisuk > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150ºC
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
MN217569
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MN217569.1
Title
Grease Waste as a Reservoir of Lipase-Producing Yeast and Description of Limtongella siamensis gen. nov., sp. nov

Author

Varunya Sakpuntoon, Jirameth Angchuan, Wanatchaporn Boontham, Pannida Khunnamwong, Chanita Boonmak, Nantana Srisuk

Abstract

A total of 175 yeast isolates were obtained from grease samples. Based on the D1/D2 region of the large subunit (LSU) ribosomal RNA (rRNA) gene analysis, 150 yeast isolates were identified as belonging to 36 described yeast species, whereas 25 isolates required more analysis. Among the described species, Rhodotorula mucilaginosa was the only Basidiomycetous yeast, whereas 149 isolates were identified as belonging to 35 described species of 15 genera in the phylum Ascomycota, and Candida tropicalis was the most abundant species. A study of lipase production indicated that strain DMKU-JMGT1-45 showed volumetric activity of 38.89 ± 9.62 and 155.56 ± 14.70 U/mL when grown in yeast extract malt extract (YM) and YM supplemented with 1% olive oil, respectively. In addition, this strain intracellularly accumulated lipid, of which the fatty acid profile revealed the major fatty acids to be 39.9% oleic acid (C18:1), 27.61% palmitoleic acid (C16:1) and 14.97% palmitic acid (C16:0). A phylogenetic analysis of the combined multi-locus gene sequences showed that the strains DMKU-JMGT1-45T and DMKU-JMGT4-14 formed a well-separated lineage and could not be assigned to any of the currently recognized genera of the Saccharomycetales. Limtongella siamensis gen. nov., sp. nov. is therefore proposed to accommodate these two strains as members of the order Saccharomycetales.

Publication Date

Link to Publication

https://doi.org/10.3390/microorganisms8010027
Publication Pdf