PYCC 9005
1 - Taxon name
Savitreella phatthalungensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
Protomyces sp.
4 - Status of the strain
Type strain of Savitreella phatthalungensis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Pumin Nutaratat et al. (Jan. 2022)
7 - Original strain number
DMKU-PAL186
8 - Accession numbers in other collections
TBRC 15109
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Pineapple leaf
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
Pineapple Field, Pa Bom District, Phatthalung Province
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
Pannida Khunnamwong, Jan. 2021
20 - Isolation details
Isolated by a dilution plate technique
21 - Deposited by
Sasitorn Jindamorakot, Oct. 2021
22 - History
Sasitorn Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
Title
A Novel Yeast Genus and Two Novel Species Isolated from Pineapple Leaves in Thailand: Savitreella phatthalungensis gen. nov., sp. nov. and Goffeauzyma siamensis sp. nov.

Author

Pumin Nutaratat, Wanatchaporn Boontham, Pannida Khunnamwong

Abstract

Four yeast strains, representing one genus and two novel anamorphic yeast species, were isolated from pineapple leaves collected in Thailand. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions showed the two strains (DMKU-PAL186 and DMKU-PAL178) were closely related to the type strains of the Protomyces and Taphrina species, but with high nucleotide divergence. Two strains (DMKU-PAL39 and DMKU-PAL18) were found to be closely related to the type strains of Goffeauzyma iberica, but with eight nucleotide substitutions in the D1/D2 domains and 26 nucleotide substitutions in the ITS regions. In phylogenetic analyses, the strains DMKU-PAL186 and DMKU-PAL178 formed a well-separated lineage from Protomyces and Taphrina genera, confirming that they represented a distinct genus, while the strains DMKU-PAL39 and DMKU-PAL18 represented a species in the genus Goffeauzyma, which was phylogenetically distinct from other recognized species of the genus. Based on molecular analyses and phenotypic characteristics, the names Savitreella gen. nov. (Taphrinomycetes, Ascomycota) and Savitreella phatthalungensis sp. nov. are proposed to accommodate the strains DMKU-PAL186 and DMKU-PAL178, and the name Goffeauzyma siamensis sp. nov. (Tremellomycetes, Basidiomycota) is proposed to accommodate the strains DMKU-PAL39 and DMKU-PAL18.

Publication Date

Link to Publication

https://doi.org/10.3390/jof8020118
Publication Pdf