PYCC 9834
1 - Taxon name
Exophiala zingiberis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Exophiala zingiberis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Rameshwar, 2023
7 - Original strain number
GH2
8 - Accession numbers in other collections
MCC 9960
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
ABS restrictions apply (PIC, MAT, IRCC, special MTA or otherwise)
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Ginger sample
14 - Category of substrate
Food & beverages
15 - Locality
Pashan, Pune
16 - Country of origin
India
17 - Latitude, longitude coordinates

18.5344, 73.7853

18 - Sample Collected by
Mrs. Yamini Nikam
19 - Isolated by and date of isolation
Ms. Srushti Shinde Apr. 23
20 - Isolation details
Infected ginger sample
21 - Deposited by
Dr. Rameshwar, Sept. 2023
22 - History
Dr. Rameshwar > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
Title
Exophiala zingiberis sp. nov., a novel cellulase-producing black yeast-like fungi isolated from ginger in India

Author

Rameshwar Avchar, Srushti Shinde, Yaminee Kashid, Parang Gangani, Avinash Sharma

Abstract

The genus Exophiala, known for its melanized, yeast-like appearance, includes a diverse group of fungi with significant implications across various fields. An isolate representing a novel species was identified within this genus from a ginger tuber from India, based on morphological characteristics and molecular phylogenetic analysis. Phylogenetic analysis of the D1/D2 domain of the 26S LSU rRNA gene, SSU rRNA gene and the internal transcribed spacer (ITS) region confirmed this strain as a new species. It was named Exophiala zingiberis sp. nov. (MycoBank no. MB 855415), with MCC 9960T designated as the holotype and PYCC 9834 as the isotype. Exophiala zingiberis was positioned as a sister species to a clade that includes Exophiala castellanii, Exophiala mali and three additional species. Phylogenetic analysis indicates that while E. zingiberis shares a common ancestor with these species, it is genetically distinct, underscoring its novel status. The type strain of this novel species differs from Exophiala yuxiensis YMF1.07354T, the most closely related species, by 19 nucleotide substitutions (3.6% sequence variation) in the D1/D2 region (549 bp compared) and 104 nucleotide substitutions and 44 gaps (27.1% sequence variation) in the ITS region (546 bp compared).

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.006614
Publication Pdf