PYCC 10006
1 - Taxon name
Starmerella kisarazuensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Starmerella kisarazuensis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Shibayama et. al. 2024
7 - Original strain number
6-K-a
8 - Accession numbers in other collections
CBS 18485; NBRC 115909
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Flowers of Red clover, Trifolium pratense
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
2-5-8, Kazusakamatari, Kisarazu-shi, Chiba
16 - Country of origin
Japan
18 - Sample Collected by
K. Shibayama, 2021
19 - Isolated by and date of isolation
K. Shibayama, 2021
20 - Isolation details
NA
21 - Deposited by
NBRC, Mar. 2024
22 - History
NBRC > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
Title
Starmerella kisarazuensis f.a., sp. nov., a novel yeast isolated from Trifolium pratense flowers

Author

Kaito Shibayama, Yumiko Miyazaki, Mai Ikeda, Kaoru Yamaguchi, Shigeki Inaba, Atsushi Yamazaki

Abstract

Three yeast isolates, NBRC 115909T, NBRC 115910 and NBRC 116270, were isolated from Trifolium pratense (red clover) flowers collected from Kisarazu, Chiba, Japan. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions revealed that these isolates represent a single novel species within the genus Starmerella. Also, no ascospore formation was observed. The yeast isolates were closely related to Starmerella vitae UWOPS 00-107.2T and Starmerella bombi NRRL Y-17081T. They differed from S. vitae, the most closely related species with a validly published name, by ten nucleotide substitutions with two gaps in the D1/D2 domains and 20 nucleotide substitutions in the ITS region. Moreover, the three isolates exhibited distinct phenotypic characteristics from the closely related species. Therefore, we suggest that these three isolates represent a novel species, designated as Starmerella kisarazuensis f.a., sp. nov. The holotype is NBRC 115909T (isotype: CBS 18485T).

Publication Date

Link to Publication

http://dx.doi.org/10.1099/ijsem.0.006253
Publication Pdf