PYCC 9704
1 - Taxon name
Wickerhamiella verensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type of Wickerhamiella verensis
5 - Basis for identification
Molecular (whole genome sequence)
6 - Identified by
Ana Pontes, 2023
7 - Original strain number
107
8 - Accession numbers in other collections
CECT 12028; CBS 10548
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
Yes
13 - Substrate of isolation
Subsoil groundwater of an old metal working factory
14 - Category of substrate
Water
15 - Locality
Vera de Bidasoa (Navarra)
16 - Country of origin
Spain
17 - Latitude, longitude coordinates

43.28085, -1.683004

18 - Sample Collected by
Menendez-Vega, D.
19 - Isolated by and date of isolation
Menendez-Vega, D.
20 - Isolation details
NA
21 - Deposited by
CECT, Mar. 2023
22 - History
CECT > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
Whole genome
ERX11798436
Title
Wickerhamiella verensis f.a. sp. nov., a novel yeast species isolated from subsoil groundwater contaminated with hydrocarbons and from a human infection

Author

Carmela Belloch, Ana Isabel Pelaez, Jesús Sánchez, Cletus P. Kurtzman

Abstract

Yeast strains belonging to a novel anamorphic yeast species were isolated from subsoil groundwater contaminated with hydrocarbons in a metal working factory located in northern Spain, and from a human infection in the USA. Comparison of ITS sequences between the isolates revealed 0.2 % divergence between the Spanish isolates and 0.46 % divergence between those and the USA isolate. Phylogenetic analysis based on the D1/D2 domains of the LSU rRNA gene showed that these isolates belong to the Wickerhamiella clade with W. sorbophila and W. infanticola as their closest relatives. Sequence divergence between the new isolates and W. sorbophila and W. infanticola was 1.97 and 1.79 %, respectively. The isolates in the novel species are not fermentative and pseudohyphae were not produced. Sexual reproduction was not observed for individual isolates or in mixtures of isolates. Conjugation between the isolates in the novel species and close relatives W. sorbophila and W. infanticola was not observed. These data support the proposal of Wickerhamiella verensis as a novel species, with CECT 12028T as the holotype.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.004053