PYCC 2405

General Information

Taxonomy

1 - Taxon name

Kazachstania pintolopesii

2 - Classification

Ascomycota

3 - Strain (species name) changes

Candida pintolopesii; Torulopsis pintolopesii; Arxiozyma telluris

4 - Status of the strain

Type strain of Kazachstania pintolopesii; Type strain of Torulopsis pintolopesii

5 - Basis for identification

Molecular (multigene)

6 - Identified by

Kurtzman et al, 2005

Other Collections

7 - Original strain number

8 - Accession numbers in other collections

ATCC 22998;CBS 1787;CCRC 21409;DBVPG 7979;IFO 0729;JCM 9834;NRRL Y-2260

Biosafety and restrictions

9 - Biological Safety Level

BSL-1

Strain details

11 - PYCC strain status

Open

12 - Mediterranean strain

Yes

13 - Substrate of isolation

liver of white mouse

14 - Category of substrate

Animal

15 - Locality

16 - Country of origin

Portugal

18 - Sample Collected by

19 - Isolated by and date of isolation

N. van Uden, 1952

20 - Isolation details

21 - Deposited by

N. van Uden, 1952

22 - History

N. van Uden > PYCC

23 - Preservation

Glass beads; 20% Glycerol; -150ºC Freeze dried ampoules

24 - Price per culture

85€

25 - Remarks

Growth conditions

26 - Medium for growth

YMA

Molecular data

DNA Sequence

Region

26S

Sequence Title

AJ276108

Gene Bank Url

http://www.ncbi.nlm.nih.gov/nuccore/AJ276108

DNA Sequence

Region

16S

Sequence Title

Y15810

Gene Bank Url

http://www.ncbi.nlm.nih.gov/nuccore/Y15810

DNA Sequence

Region

26S

Sequence Title

AY545574

Gene Bank Url

http://www.ncbi.nlm.nih.gov/nuccore/AY545574

Phenotype

Fermentation

F1 D-Glucose

W, D

F2 D-Galactose

F3 Maltose

F5 Sucrose

F6 a,a-Trehalose

F7 Melibiose

F8 Lactose

F9 Cellobiose

F10 Melezitose

F11 Raffinose

F12 Inulin

Assimilation-Growth

C1 D-Glucose

C2 D-Galactose

C3 L-Sorbose

C4 D-Glucosamine

C5 D-Ribose

C6 D-Xylose

C7 L-Arabinose

C8 D-Arabinose

C9 L-Rhamnose

C10 Sucrose

C11 Maltose

C12 a,a-Trehalose

C13 Me a-D-Glucoside

C14 Cellobiose

C15 Salicin

C16 Arbutin spliting

C17 Melibiose

C18 Lactose

C19 Raffinose

C20 Melezitose

C21 Inulin

C22 Starch

C23 Glycerol

C24 Erythritol

C25 Ribitol

C26 Xylitol

C28 D-Glucitol

C29 D-Mannitol

C30 Galactitol

C31 myo-Inositol

C32 D-Glucono-1,5-lactone

C35 D-Gluconate

C36 D-Glucuronate

C38 DL-Lactate

C39 Succinate

C40 Citrate

C41 Methanol

C42 Ethanol

C43 Propane 1,2 diol

C44 Butane 2,3 diol

C46 D-glucarate

C50 L-Malic acid

C51 L-Tartaric acid

N1 Nitrate

N2 Nitrite

N3 Ethylamine

N4 L-Lysine

N5 Cadaverine

N6 Creatine

N7 Creatinine

V1 w/o vitamins

T1 Growth at 25ºC

T1 Growth at 30ºC

T3 Growth at 35ºC

T4 Growth at 37ºC

T5 Growth at 40ºC

T6 Growth at 42ºC

T7 Growth at 45ºC

O1 Cycloheximide 0.01%

O2 Cycloheximide 0.1%

O3 Acetic acid 1%

O4 50% D-Glucose

O6 10% NaCl

M1 Starch formation

M3 Urea hydrolysis

M4 Diazonium Blue B reaction

Bibliography

Title

Multigene phylogenetic analysis of pathogenic candida species in the Kazachstania (Arxiozyma) telluris complex and description of their ascosporic states as Kazachstania bovina sp. nov. K. heterogenica sp. nov. K. pintolopesii sp. nov. and K. slooffiae

Author

Cletus P. Kurtzman, Christie J. Robnett, Jerrold M. Ward, Cory Brayton, Peter Gorelick, Thomas J. Walsh

Abstract

ABSTRACT A yeast causing widespread infection of laboratory mice was identified from 26S rRNA gene sequences as Candida pintolopesii . To determine the relationship of C. pintolopesii with other members of the Kazachstania ( Arxiozyma ) telluris species complex, nucleotide sequences from domains 1 and 2 of the 26S rRNA gene, the mitochondrial small-subunit rRNA gene, and the RNA polymerase II gene were phylogenetically analyzed. That analysis resolved the 48 strains examined into five closely related species: K. telluris , Candida bovina , C. pintolopesii , Candida slooffiae , and a previously unknown species. One or more strains of each of the last four species formed an ascosporic state much like that of K. telluris . To place these ascosporogenous strains taxonomically, it is proposed that they be assigned to the teleomorphic genus Kazachstania as K. bovina (type strain NRRL Y-7283, CBS 9732, from the nasal passage of a pigeon), K. heterogenica (type strain NRRL Y-27499, CBS 2675, from rodent feces), K. pintolopesii (type strain NRRL Y-27500, CBS 2985, from the peritoneal fluid of a dead guinea pig), and K. slooffiae (type strain NRRL YB-4349, CBS 9733, from the cecum of a horse). On the basis of multigene sequence analyses, K. heterogenica appears to be a hybrid of K. pintolopesii and a presently unknown species. With the exception of K. bovina , the phylogenetically defined species show a moderate degree of host specificity.

Publication Date

05 Jan 2005

Link to Publication

http://dx.doi.org/10.1128/jcm.43.1.101-111.2005