PYCC 9982
1 - Taxon name
Symmetrospora pini
2 - Classification
Basidiomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Symmetrospora pini
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
S. Liu , May.2023
7 - Original strain number
NYNU 232201
8 - Accession numbers in other collections
CICC 33601
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Leaf
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
Pingtang, Guizhou
16 - Country of origin
China
17 - Latitude, longitude coordinates

25.1166666667, 107.033333333

18 - Sample Collected by
D. Lu
19 - Isolated by and date of isolation
S. Liu , Mar.2023
20 - Isolation details
YMA with chloramphenicol, 20ºC
21 - Deposited by
Feng-Li, Jan. 2024
22 - History
Fen-Li > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
MYP
DNA Sequence
Region
26S
Sequence Title
OQ851926
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/OQ851926
DNA Sequence
Region
ITS
Sequence Title
OQ851927
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/OQ851927
Title
Symmetrospora pini sp. nov., an anamorphic yeast species isolated from plant leaves

Author

Peng Wang, Chun-Yue Chai, Feng-Li Hui

Abstract

Two red yeast strains, NYNU 232201 and NYNU 232218, were isolated from the phylloplane of Pinus massoniana and Cinnamomum camphora, respectively, in Sifangjing Village, Pingtang County, Guizhou Province, China. Molecular phylogenetic analyses placed these strains in close relation to Symmetrospora clarorosea, Symmetrospora gracilis and Symmetrospora eucalyti. Genetically, the isolates differed from the type strains of these related species by seven to nine base substitutions (1.2–1.5%) in the D1/D2 domain and by more than 15 base mismatches (2.6%) in the internal transcribed spacer region. Phenotypically, the two strains can be distinguished from S. clarorosea and S. gracilis by their ability to assimilate d-glucosamine and grow at 30 °C, as well as their inability to assimilate ribitol. Physiological data for S. eucalypti were not available for comparison. No sexual morph was observed in either strain. Based on molecular and phenotypic differentiation, the novel yeast species Symmetrospora pini sp. nov. is proposed with holotype CICC 33601T (MycoBank MB 857729).

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.006812
Publication Pdf